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姜永厚 性别:男 出生年月:1971年4月 职称:副教授 所在学科:微生物 办公室:6号楼433室 电话:15257168248 电子邮件:yonghoujiang@163.com |
主要学习工作经历
2012.04-2013.05美国爱荷华州立大学 访问学者
2011.02-2011.07上海外国语大学 出国英语培训
2004.06-至今 浙江理工大学 教学科研
2001.09-2004.06浙江大学 博士生
1998.09-2001.07扬州大学 硕士生
1994.08-1998.08江苏连云港如意集团公司 技术员
1990.09-1994.07扬州大学 本科生
主要研究方向
1.动物病毒分子诊断
2.基因芯片技术
3.动物病毒分子生物学
获奖与荣誉
1.浙江省优秀科技特派员,2011年
2.浙江理工大学优秀共产党员,2010年
3.浙江省科学技术二等奖,2010年
4.校优秀硕士学位论文指导教师,2011年
科研教学项目
1.浙江省自然科学基金项目(LY15C010006),一种新的猪圆环病毒2型转录物分析及其功能研究,2015.01-2017.12,主持
2.浙江理工大学研究生课程建设项目,《高级生态学》课程建设(YKC-Z15006),2015.06-2017.6,主持
3.浙江省高校生物学重中之重学科优秀青年人才培养基金(2014C08),基于熔解曲线分析EvaGreen荧光定量PCR同时检测多种病毒方法的应用研究,2014.05-2016.05,主持
4.浙江省自然科学基金,猪圆环病毒2型ORF4转录和功能分析(Y3090166),2010.01-2012.12.主持
5.浙江省科技攻关面上重点项目,一种新的基因芯片检测技术的开发应用研究(2008C22081),2008.01-2010.12.主持
6.浙江省高等学校优秀青年教师资助计划,2010.09-2011.09.主持
7.浙江省研究生创新科研项目(YK2011057),2011.12-2013.12指导老师 主持
8.浙江省“生物医学工程”重中之重学科开放基金,基于LDR新型通用寡核苷酸芯片检测多种猪病毒研究(SWYX0908),2009.10-2010.09.主持
9.浙江理工大学科研基金,基因芯片技术检测几种猪病毒研究(0616274-Y),2007.01-2009.12.主持
10.浙江理工大学高等教育教学改革研究项目,生态学教学改革与实践(YB07027),2007.07-2009.07.主持
11.浙江省科技攻关重点项目,猪多病原混合感染的基因诊断与高通量快速检测技术研究(2006C12039),2005.01-2008.6.排名2(校内1)
12.浙江省自然科学基金项目,苜蓿叶绿体高效表达无抗生素标记的猪瘟植物口服疫苗研究(LY15C020005),2015.01-2017.12.参加(排名4)
13.国家自然科学基金项目,猪圆环病毒2型感染中NF-κB信号通路的激活机制及其对细胞凋亡的作用研究(31101831),2012.01-2014.12.排名2
14.浙江省自然科学基金,农药氟虫腈作用下斑马鱼miR-155对cyb561d2的调控作用研究(1204637-D),2012.07-2013.12.排名2
15.浙江省科技厅项目,耐辐射奇球菌抗辐射相关非编码小RNA及其靶标mRNA的鉴定(1204347-E),2012.04-2014.03.排名2
16.浙江省自然科学基金,用衣藻叶绿体高效表达禽流感饲用疫苗研究(Y3090487),2010.1-2012.12.排名2
主要专著论文
1.Zheng Xiaowen#, Liu Gaopeng#, Opriessnig Tanja, Wang Zining, Yang Zongqi,Jiang Yonghou (通讯作者). 2016. Rapid detection and grouping of porcine bocaviruses by an EvaGreen® based multiplex real-time PCR assay using melting curve analysis. Molecular and Cellular Probes, 10.1016/j.mcp.2016.05.001. (IF2015=1.85)
2.Zheng Xiaowen, Liu Gaopeng, Opriessnig Tanja, Wang Zining, Yang Zongqi,Jiang Yonghou (通讯作者). 2016. Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses. Journal of Virological Methods, Accepted (IF2015=1.78)
3.郑晓文#,饶品彬#,蔡晔,杨宗歧,姜永厚(通讯作者). 2016. EvaGreen实时荧光PCR检测PRRSV方法的建立和应用.江苏农业科学, 录用
4.吴海港,饶品彬,蔡晔,全滟平,姜永厚(通讯作者). 2015.快速实时荧光定量PCR检测猪繁殖与呼吸综合症病毒方法的建立.浙江理工大学学报,33(2): 260-265
5.Rao Pinbin, Wu Haigang,Jiang Yonghou (通讯作者),Opriessnig Tanja, Zheng Xiaowen, Mo Yecheng,Yang Zongqi.2014. Development of an EvaGreen-based multiplex real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of six viral pathogens of porcine reproductive and respiratory disorder. Journal of Virological Methods, 208: 56-62 (IF2013=1.88)
6.Wu Haigang, Rao Pinbin,Jiang Yonghou(通讯作者),Opriessnig Tanja, Yang Zongqi. 2014.A sensitive multiplex real-time PCR panel for rapid diagnosis of viruses associated with porcine respiratory and reproductive disorders. Molecular and Cellular Probes, 28(5-6): 264-270 (IF2013=1.86)
7.Gao Zhangzhao, Dong Qinfang,Jiang Yonghou(通讯作者), Opriessnig Tanja, Wang Jingxiu, Quan Yanping, Yang Zongqi. 2014. ORF4-protein deficient PCV2 mutants enhance virus-induced apoptosis and show differential expression of mRNAs in vitro. Virus Research, 183: 56-62. (IF2013=2.83)
8.Luis Giménez-Lirola,Jiang Yonghou, Sun Dong, Hoang Hai, Yoon Kyoung-Jin, Halbur Patrick G., Opriessnig Tanja. 2014. Simultaneous detection of antibodies against Apx toxins ApxI, ApxII, ApxIII, and ApxIV in pigs with known and unknown Actinobacillus pleuropneumoniae exposure using a multiplexing liquid array platform. Clinical and Vaccine Immunology, 21(1): 85-95. (IF2012=2.60)
9.Jiang Yonghou, Xiao Chaoting, Yin Shuanghui, Gerber Priscilla F., Opriessnig Tanja, Halbur Patrick G. 2014. High prevalence and genetic persity of porcine bocaviruses in pigs in theUSA, and identification of multiple novel porcine bocaviruses. Journal of General Virology, 95(2): 453-65. (IF2013=3.53)
10.蔡晔,饶品彬,吴海港,姜永厚(通讯作者). 2014. EvaGreen实时荧光定量PCR方法检测猪圆环病毒2型方法的建立.生物技术通报,6: 77-82
11.Gao Zhangzhao, Dong Qinfang,Jiang Yonghou(通讯作者),Opriessnig Tanja, Wang Jingxiu, Quan Yanping, Yang Zongqi. 2013. Identification and characterization of two novel transcription units of porcine circovirus 2. Virus Genes, 47(2): 268-275. (IF2013=1.84)
12.Cheng Juhui,Jiang Yonghou(通讯作者), Rao Pinbin, Wu Haigang, Dong Qinfang, Wu Zhiyi, Ding Xianfeng, Guo Jiangfeng. 2013. Development of a single-tube multiplex real-time PCR for detection and identification of five pathogenic targets by using melting-curve analysis with EvaGreen. Archives of Virology, 158(2): 379-386. (IF2013=2.28)
13.王净修,高章照,姜永厚(通讯作者),吴海港,饶品彬,全滟平,徐辉. 2014.猪圆环病毒2型ORF4基因的原核表达及多克隆抗体的制备.浙江农业学报,26(1): 34-39.
14.Xiao Chaoting, Giménez-Lirola Luis,Jiang Yonghou, Halbur Patrick G., Opriessnig Tanja. 2013. Characterization of a novel porcine parvovirus tentatively designated PPV5. PLoS ONE, 8(6): e65312. (IF2012=3.73)
15.姜永厚,丁海琴,陆秋萍,王尧骏,盛清,郭江峰. 2013.生物类专业本科生导师制实施现状与对策.高校生物学教学研究(电子版),2(2):11-14.
16.Xiao Chaoting, Giménez-Lirola Luis, Gerber Priscilla,Jiang Yonghou, Halbur Patrick G., Opriessnig Tanja. 2012. Identification and characterization of novel porcine astroviruses (PAstV) with high prevalence and frequent co-infection of inpidual pigs with multiple PAstV types. Journal of General Virology, 94(3): 570-82. (IF2011=3.36)
17.Jiang Yonghou, Guo Yao, Wang Ping, Dong Qinfang, Opriessnig Tanja, Xu Hui, Cheng Juhui, Ding Xianfeng, Guo Jiangfeng. 2011. A novel diagnostic platform based on multiplex ligase detection -PCR and microarray for simultaneous detection of swine viruses. Journal of Virological Methods, 178(1-2): 171-178. (IF2011=2.14)
18.Wang Ping, Guo Yao, Cheng Juhui, Dong Qinfang, Ding Xianfeng, Guo Jiangfeng,Jiang Yonghou(通讯作者). 2011. Development of multiplex reverse transcription-ligase detection reaction-polymerase chain reaction (MRLP) mediated universal DNA microarray for diagnostic platform. Biosensors and Bioelectronics, 26(8): 3719-3724.(IF2011=5.43)
19.Wang Ping, Guo Yao, Cheng Juhui, Dong Qinfang, Ding Xianfeng, Guo Jiangfeng,Jiang Yonghou(通讯作者). 2012. Application of multiplex reverse transcription-ligase detection reaction-polymerase chain reaction (MRLP) mediated universal DNA microarray for detecting potato viruses in field samples. European Journal of Plant Pathology, 132(2): 217-227.(IF2012=1.61)
20.郭瑶,汪平,董沁芳,程菊会,徐辉,丁先锋,郭江峰,姜永厚(通讯作者).2011.连接酶检测反应(LDR)-PCR基因芯片同时检测猪圆环病毒2型、猪细小病毒和伪狂犬病毒.中国预防兽医学报,33(7): 526-530.
21.董沁芳,郭瑶,汪平,程菊会,徐辉,丁先锋,郭江峰,姜永厚(通讯作者). 2012.猪细小病毒LDR-PCR检测方法的建立和应用.生物技术通报,2: 165-169.
22.Jin Youfang, Xu Genming, Li Yan, Meng Li,Jiang Yonghou, Guo Jiangfeng, Ding Xianfeng. 2011. A Novel normalization approach for microRNA quantitative PCR. Progress in Biochemistry and Biophysics, 38(5): 473-481.(IF2011=0.24)
23.汪平,郭瑶,程菊会,董沁芳,商晗武,姜永厚(通讯作者). 2011.马铃薯卷叶病毒的RT-LDR-PCR检测.植物保护学报,38(5): 471-272
24.Jiang Yonghou, Shang Hangwu, Xu Hui, Ding Xianfeng, Chen Weijie, Zhao Lingyan, Fang Li. 2010. Detection and genotyping of porcine circovirus in naturally infected pigs by oligo-microarray. Research in Veterinary Science, 89 (1): 133-139. (IF2011=1.33)
25.姜永厚,商晗武,徐辉,陈伟杰,赵灵燕,丁先锋. 2010.基于多重PCR的寡核苷酸芯片检测猪圆环病毒,猪瘟病毒和猪繁殖与呼吸障碍综合征病.中国预防兽医学报,32(3):194-199.
26.Jiang Yonghou, Shang Hanwu, Xu Hui, Zhu Liangjun, Chen Weijie, Zhao Lingyan, Fang Li. 2010. Simultaneous detection of porcine circovirus-2, classical swine fever virus, porcine parvovirus, and porcine reproductive and respiratory syndrome virus in pig by multiplex polymerase chain reaction. The Veterinary Journal, 183(2): 172-175. (IF2009=2.79)
27.姜永厚,商晗武,徐辉,陈伟杰,丁先锋,赵灵燕. 2009.猪瘟病毒基因芯片检测技术的建立与应用.浙江农业学报,21(2):85-90.
28.姜永厚,商晗武,徐辉,陈伟杰,赵灵燕,丁先锋. 2008.猪圆环病毒检测和分型寡核苷酸芯片的建立和应用.中国兽医学报,28(10):1174-1180.
29.陈集双,姜永厚,主编. 2006.外来入侵生物控制.浙江大学出版社.
国家发明专利
1.姜永厚,吴海港,饶品彬.荧光定量PCR检测猪呼吸繁殖障碍疾病相关病毒试剂盒,101410465637.8,2016年授权
2.姜永厚,吴海港,饶品彬.荧光定量PCR检测猪主要病毒的试剂盒,201410466604.5,2016年授权
3.姜永厚,程菊会,董沁芳,郭江峰,丁先锋.多重实时荧光PCR同时检测五种马铃薯病毒的方法,ZL201210080834.9,2013年授权
4.姜永厚,徐辉,商晗武,竺锡武,赵灵燕,陈伟杰,丁先锋.同时检测多种混合感染病毒的基因芯片,ZL200810059596.7,2011年授权
5.姜永厚,郑晓文,刘高鹏.猪博卡病毒检测和分型富集多重PCR快速诊断试剂盒,201610244838.4,申请受理中
6.姜永厚,郑晓文,刘高鹏.猪博卡病毒实时荧光定量PCR检测和分型诊断试剂盒,201610257262.5,申请受理中